The last living members of an extinct species Jan Stejskal

In the savannahs of Kenya, two female
northern white rhinos, Nájin and Fatu,

munch contentedly on the grass.

At the time of this video’s publication,

these are the last two known
northern white rhinos left on Earth.

Their species is functionally extinct—

without a male,
Nájin and Fatu can’t reproduce.

And yet, there’s still hope to revive
the northern white rhino.

How can that be?

The story starts about 50 years ago,

when poachers began illegally hunting
thousands of rhinos

across Africa for their horns.

This, combined with civil wars
in their territory,

decimated northern white rhino
populations.

Concerned conservationists began trying
to breed them in captivity in the 1970s,

collecting and storing semen from males.

Only four rhinos were ultimately born
through the ambitious breeding program.

Nájin, and her daughter Fatu
were the last two.

In 2014, conservationists discovered
that neither can have a calf.

Though Nájin gave birth to Fatu,
she now has weak hindlegs,

which could harm her health
if she became pregnant again.

Fatu, meanwhile,
has a degenerated uterine lining.

Then, the last northern white rhino male
of the species, Sudan, died in 2018.

But there was one glimmer of hope:
artificial reproduction.

With no living males and no females
able to carry a pregnancy,

this is a complicated and risky process
to say the least.

Though scientists had stored semen,
they would have to collect the eggs—

a complex procedure that requires a female
to be sedated for up to two hours.

Then, they’d create a viable embryo
in the lab—

something that had never been done before,
and no one knew how to do.

Even that was just the beginning—

a surrogate mother
of another rhino species

would have to carry the embryo to term.

Females of a closely related species,
the southern white rhino,

became both the key to developing
a rhino embryo in a lab

and the leading candidates
for surrogate mothers.

Northern and southern white rhinos
diverged about a million of years ago

into separate— though still
closely-related— species.

They inhabit different regions, and have
slightly different physical traits.

In a fortunate coincidence,
several female southern white rhinos

needed treatment
for their own reproductive problems,

and researchers could collect eggs
as part of that treatment.

In Dvůr Králové Zoo in October 2015,

experts of IZW Berlin began collecting
eggs from southern white rhinos

and sending them to Avantea, an animal
reproduction laboratory in Italy.

There, scientists developed and perfected
a technique to create a viable embryo.

Once they mastered the technique,

researchers extracted Nájin and Fatu’s
eggs on August 22, 2019

and flew them to Italy.

Three days later, they fertilized
the eggs with sperm

from a northern white rhino male.

After another week, two of the eggs
made it to the stage of development

when the embryo can be frozen
and preserved for future.

Another collection in December 2019
produced one more embryo.

As of early 2020, the plan is to collect
Nájin and Fatu’s eggs three times a year

if they’re healthy enough.

In the meantime,
researchers are looking for

promising southern white rhino
surrogate mothers—

ideally who’ve carried a pregnancy
to term before.

The surrogacy plan
is somewhat of a leap of faith—

southern and northern white rhinos
have interbred

both during the last glacial period
and more recently in 1977,

so researchers are optimistic
a southern white rhino

would be able to carry
a northern white rhino to term.

Also, the two species’ pregnancies
are the same length.

Still, transferring an embryo
to a rhino is tricky

because of the shape of the cervix.

The ultimate goal,
which will take decades,

is to establish a breeding population
of northern white rhinos

in their original range.

Studies suggest that we have samples
from enough individuals

to recreate a population with the genetic
diversity the species had a century ago.

Though the specifics
of this effort are unique,

as more species face critical endangerment
or functional extinction,

it’s also an arena for big questions:

do we have a responsibility to try
to bring species back from the brink,

especially when human actions
brought them there in the first place?

Are there limits to the effort
we should expend

on saving animals threatened
with extinction?

在肯尼亚的大草原上,两只雌性
北方白犀牛 Nájin 和 Fatu

心满意足地在草地上咀嚼。

在这段视频发布时,

这是地球上最后两只已知的
北方白犀牛。

他们的物种在功能上已经灭绝——

没有雄性,
纳金和法图就无法繁殖。

然而,仍有希望
重振北方白犀牛。

怎么可能?

故事开始于大约 50 年前,

当时偷猎者开始在非洲非法猎杀
数千头犀牛

以获取犀牛角。

再加
上其领土内的内战,

北方白犀牛
种群数量锐减。 1970 年代,

有关的环保主义者开始
尝试圈养它们,

收集和储存雄性的精液。 通过雄心勃勃的育种计划

,最终只有四头犀牛诞生

Nájin 和她的女儿 Fatu
是最后两个。

2014 年,环保主义者
发现两者都不可能有小牛。

纳金虽然生下了法图,
但她现在的后腿很弱,如果她再次怀孕

可能会损害她的健康

与此同时,法
图的子宫内膜退化。

然后,该物种的最后一只雄性北方白犀牛
苏丹于 2018 年去世。

但有一线希望:
人工繁殖。

由于没有活着的男性和女性
能够怀孕,至少可以说

这是一个复杂而危险的
过程。

尽管科学家们已经储存了精液,
但他们必须收集卵子——这

是一个复杂的过程,需要对
雌性进行长达两个小时的镇静。

然后,他们将在实验室中创造出一个可行的胚胎
——

这是以前从未做过的事情,
也没有人知道该怎么做。

即便这只是开始

——另一种犀牛物种

的代孕母亲必须将胚胎移植到足月。

一个密切相关的
物种南方白犀牛的雌性

成为在实验室中培育犀牛胚胎的关键,

也是代孕母亲的主要候选人。 大约一百万年前,

北方和南方的白犀牛
分化

为独立的——尽管仍然
密切相关的——物种。

它们栖息在不同的地区,
身体特征略有不同。

幸运的是,
几头雌性南方白犀牛

因自身的生殖问题需要治疗

,研究人员可以收集卵
作为治疗的一部分。

2015 年 10 月,在 Dvůr Králové 动物园

,IZW 柏林的专家开始
收集南方白犀牛的卵

,并将它们送到意大利的动物
繁殖实验室 Avantea。

在那里,科学家们开发并完善
了一种创造可行胚胎的技术。

一旦掌握了这项技术,

研究人员
就于 2019 年 8 月 22 日提取了 Nájin 和 Fatu 的卵,

并将它们空运到意大利。

三天后,他们

用一只雄性北方白犀牛的精子使卵子受精。

又过了一周,其中两个
卵子进入发育阶段

,胚胎可以
冷冻保存以备将来使用。

2019 年 12 月的另一个系列又
产生了一个胚胎。

从 2020 年初开始,如果
Nájin 和 Fatu 的卵足够健康,计划每年收集 3 次

与此同时,
研究人员正在寻找

有前途的南方白犀牛
代孕妈妈——

最好是那些已经怀孕
到足月的代孕妈妈。

代孕计划
在某种程度上是一种信仰的飞跃——

南方白犀牛和北方白犀牛

在上一次冰期
和最近的 1977 年都进行了杂交,

因此研究人员乐观地认为
南方白犀牛

能够
将北方白犀牛孕育到足月。

此外,这两个物种的怀孕
长度相同。

尽管如此,由于子宫颈的形状,将胚胎移植
到犀牛身上还是很棘手

的。

最终目标是在其原始范围内建立北方白犀牛的繁殖种群
,这将需要数十年的时间

研究表明,我们有
足够多的个体样本

来重建具有
该物种一个世纪前遗传多样性的种群。

尽管这项努力的细节是独一无二的,

随着更多物种面临严重的濒危
或功能性灭绝,

这也是一个重大问题的舞台:

我们是否有责任
试图将物种从边缘带回来,

尤其是当人类行为
将它们带入边缘时 第一名?

我们应该

为拯救
濒临灭绝的动物付出的努力是否有限制?